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18年专注胆汁酸对脂肪利用和肝肠健康的研究

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又一篇SCI论文!证明了日粮中添加胆汁酸对舌鳎肝脏健康的有益影响!

Dietary Supplementation of Bile Acids in Tongue Sole (Cynoglossus semilaevis): A Promising Strategy to Improve Hepatic Health Status

半滑舌鳎日粮中补充胆汁酸:一个有希望改善肝脏健康状况的策略

作者:王升鹏、张伟、曹爱智、潘中超、刘太亮

A 10-week feeding trial was conducted to investigate the beneficial effects of bile acids (BAs) on hepatic health status in tongue sole (Cynoglossus semilaevis). Three experimental diets were prepared with different levels of BA inclusion in the commercial basal diet: control group (CT: 0 mg/kg basal diet), low-level group (BA1: 300 mg/kg basal diet), and high-level group (900 mg/kg basal diet). At the end of the feeding trial, growth performance and survival rate were measured, as well as numerous physiological and biochemical parameters of the liver, including four hepatic function indices, five antioxidant indices, five lipid metabolism parameters, and three digestive enzyme activities. Moreover,the mRNA expression levels of three growth-related genes and three immune-related genes in the liver were assayed. Results showed that growth performance and survival were substantially improved in both low- and high-level BA inclusive groups. Further, the tested liver physiological and biochemical parameters combined with the quantitative real-time PCR results revealed enhanced antioxidant capacity, energy metabolism, digestive ability, and immune response. Finally, these findings provide a wider spectrum of the beneficial effects of dietary BAs on liver health in tongue sole.

译文:

为研究胆汁酸对半滑舌鳎(Cynoglossus semilaevis)肝脏健康状况的有益影响,进行了为期10周的喂养试验。在商业基础日粮中制备了三种含有不同水平胆汁酸的试验日粮:对照组(0 mg/kg)、低水平组(300 mg/kg)和高水平组(900 mg/kg)。喂食试验结束后,测量生长性能和存活率,以及肝脏的各项生理生化参数,包括四项肝功能指标、五项抗氧化指标、五项脂代谢参数和三项消化酶活性。此外,还检测了肝脏中三个生长相关基因和三个免疫相关基因的mRNA表达水平。结果表明,低水平胆汁酸组和高水平胆汁酸组的生长性能和存活率均显著提高。此外,检测的肝脏生理生化参数结合实时定量PCR结果显示:抗氧化能力、能量代谢、消化能力和免疫反应增强。最后,这些发现提供了更广泛的日粮添加胆汁酸对舌鳎肝脏健康的有益影响。

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